Reporter

Part:BBa_K1093020

Designed by: Jakub Piątkowski   Group: iGEM13_Warsaw   (2013-09-21)


HBA+NsfGFP (+B0034)

Human hemoglobin alpha chain fused at C-terminus with the N-terminal fragment of superfolder green fluorescent protein. Along with BBa_K1093021 and BBa_K1093031 (hemoglobin beta chain fused with two variants of C-terminal of sfGFP) it is intended as a part of a reporter system for acrylamide detection. Formation of adducts on the N-terminal valine of both hemoglobin chains is known to ihibit proper assembly of hemoglobin tetramer. Part already contains a strong RBS (BBa_B0034). The coding sequence has been codon optimised for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 509


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Categories
Parameters
None